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    BioThermD- Taq DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 BioThermD- Taq DNA Polymerase

 GC-064-0100  

 100 u

 11 

 BioThermD- Taq DNA Polymerase

 GC-064-0250  

 250 u

 27.5 

 BioThermD- Taq DNA Polymerase

 GC-064-0500  

 500 u

 55 

 BioThermD- Taq DNA Polymerase

 GC-064-1000  

 1000 u

 110 

 BioThermD- Taq DNA Polymerase

 GC-064-5000  

 5000 u

 550 



  DESCRIPTION
BioThermD- DNA polymerase is a special purified DNA polymerase without any traces of E. coli DNA. It is optimal for the amplification and sequencing of 16S rRNA genes to identify bacteria.

  CONCENTRATION
5 units/l

  UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25C); 50 mM KCl; 2 mM MgCl2; 1 mM -mercaptoethanol) and activated calf thymus DNA as substrate.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol(v/v)

  STORAGE TEMPERATURE
Store BioThermD- DNA polymerase below 0C, preferably at -20C, in a constant temperature freezer.

  10X REACTION BUFFER
160 mM (NH4) 2SO4, 670 mM Tris-HCl pH 8.8 (at 25C), 15 mM MgCl2, 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.

1.5 ml 10x reaction buffer (contains 15 mM MgCl2) Cat. No. GC-002-006

1.5 ml 10x reaction buffer without MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007

  




 
 
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