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    BioThermRed™ Taq DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 BioThermRed™ Taq DNA Polymerase

 GC-021-0100  

 100 u

 11.5 

 BioThermRed™ Taq DNA Polymerase

 GC-021-0250  

 250 u

 28.75 

 BioThermRed™ Taq DNA Polymerase

 GC-021-0500  

 500 u

 57.5 

 BioThermRed™ Taq DNA Polymerase

 GC-021-1000  

 1000 u

 115 

 BioThermRed™ Taq DNA Polymerase

 GC-021-5000  

 5000 u

 575 



  DESCRIPTION
BioThermRed™ DNA polymerase is a mixture of BioTherm™ DNA polymerase and red pigment that works like BioTherm™. After addition of BioThermRed™ a thin red layer on the bottom of the tube appears. So you have a visible pipetting control. Furthermore you can see, if your reaction is already mixed, when the color of your reaction becomes uniform.

  BESCHREIBUNG
BioThermRed™ ist eine neue, mit rotem Farbstoff versehene Variante der BioTherm™ DNA Polymerase. Sie funktioniert genauso wie BioTherm™ DNA Polymerase. Nach Zugabe von BioThermRed™ erscheint eine dünne rote Schicht am Boden des Reaktionsgefäßes. Dadurch haben Sie eine deutlich sichere Pipettierkontrolle. Wei-terhin können Sie anhand der roten Farbe erkennen, ob ihr Ansatz gut durchmischt ist. Gleichmäßige Farb-verteilung deutet auf eine vollkommene Durchmischung der Probe hin.

  CONCENTRATION
5 units/µl

  UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form
in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic
acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20,0.2% indicator red dye, 50% glycerol (v/v) The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.

  STORAGE TEMPERATURE
Store BioThermRed™ DNA Polymerase below 0ºC, preferably at -20°C, in a constant temperature freezer.

  10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl2, 0.1% Tween 20

1.5 ml 10x reaction buffer (contains 15 mM MgCl2) Cat. No. GC-002-006

1.5 ml 10x reaction buffer without MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007

  ASSOCIATED ACTIVITIES
Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of
1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72°C in the presence of 15-
20 units of BioThermRed™ DNA Polymerase.

  COMPANION PRODUCTS
BioTherm™ DNA Polymerase, SupraTherm™ DNA Polymerase, dNTPs

  



 
 
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