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    BioThermStar™ Hot Start Taq DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 BioThermStar™ Hot Start Taq DNA Polymerase

 GC-045-0100  

 100 u

 13 

 BioThermStar™ Hot Start Taq DNA Polymerase

 GC-045-0250  

 250 u

 32.5 

 BioThermStar™ Hot Start Taq DNA Polymerase

 GC-045-0500  

 500 u

 65 

 BioThermStar™ Hot Start Taq DNA Polymerase

 GC-045-1000  

 1000 u

 130 

 BioThermStar™ Hot Start Taq DNA Polymerase

 GC-045-5000  

 5000 u

 650 



  DESCRIPTION
BioThermStar™ is a thermostable DNA polymerase purified from the Thermus aquaticus strain in accordance with the procedures developed by Kaledin for the isolation of thermostable enzymes processing DNA polymerase activity from thermophilic bacteria. BioThermStar™ is a modified form of the enzyme designed for Hot-Start-PCR. It is a highly processive 5'-3' DNA polymerase lacking 3'-5'-exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- or exonucleases.

  BUFFER pH
We strongly recommend to use buffers at pH 8.3 with a concentration of 1,5mM MgCl2!

  ACTIVATION
This polymerase is inactive until incubated 7-10 min at 95°C!!! These activation conditions are extremely important!!! The activation completely prevents nonspecific primer annealing and the formation of primer-dimers during setup.

  REACTION
It is also very important to pay great attention to the actual temperature (95°C) inside the tube!!!

  APPLICATION
Hot-Start-PCR
PCR with high specificity

  CONCENTRATION
5 units/µl

  UNIT DEFINITION
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 min at 72°C.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)

  STORAGE TEMPERATURE
-20°C

  10X REACTION BUFER
160 mM (NH 4 ) 2 SO 4 , 670 mM Tris-HCl pH 8.3 (at 25°C), 15 mM MgCl 2 , 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
Please note the difference between BioTherm ™ and BioThermStar ™ buffers!

  QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.

  COMPANION PRODUCTS
BioThermAB™, MagicTubes™

  BioThermStar IN A TAQMAN ASSAY
BioThermStar IN A TAQMAN ASSAY

  




 
 
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