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    BioThermBio™ Taq DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 BioThermBio™ Taq DNA Polymerase

 GC-057-0100  

 100 u

 20 

 BioThermBio™ Taq DNA Polymerase

 GC-057-0250  

 250 u

 50 

 BioThermBio™ Taq DNA Polymerase

 GC-057-0500  

 500 u

 100 

 BioThermBio™ Taq DNA Polymerase

 GC-057-1000  

 1000 u

 200 

 BioThermBio™ Taq DNA Polymerase

 GC-057-5000  

 5000 u

 1000 



  DESCRIPTION
BioThermBio™ is a novel thermostable DNA polymerase that dramatically improves incorporation of Biotin- and Digoxigenin-dUTP as compared to Taq DNA polymerase.

  BESCHREIBUNG
BioThermBio™ ist eine neue hitzestabile DNA Polymerase, welche den Einbau von Biotin- und Digoxigenin-dUTP in DNA grundlegend verbessert.

  CONCENTRATION
5 units/µl

  UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72ºC under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25ºC); 50 mM KCl; 2 mM MgCl2; 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol(v/v)

  STORAGE TEMPERATURE
Store BioThermBio™ DNA polymerase below 0ºC, preferably at -20ºC, in a constant temperature freezer.

  10X REACTION BUFER
160 mM (NH4)2SO4 , 670 mM Tris-HCl pH 8.8 (at 25ºC), 15 mM MgCl2, 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.

1.5 ml 10x reaction buffer (contains 15 mM MgCl2) Cat. No. GC-002-006

1.5 ml 10x reaction buffer without MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007

  ASSOCIATED ACTIVITIES
Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of 1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72ºC in the presence of 15 - 20 units of BioTherm™ DNA polymerase.

  COMPANION PRODUCTS
BioThermRed™ DNA polymerase, SupraTherm™ DNA polymerase, dNTPs, T-Vector

  PICTURE 1
PICTURE 1

  



 
 
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