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    RNase-Inhibitor

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 RNase-Inhibitor

 GC-042-0100  

 100 u

 5 

 RNase-Inhibitor

 GC-042-0250  

 250 u

 12.5 

 RNase-Inhibitor

 GC-042-0500  

 500 u

 25 

 RNase-Inhibitor

 GC-042-1000  

 1000 u

 50 

 RNase-Inhibitor

 GC-042-5000  

 5000 u

 250 



  DESCRIPTION
Native RNase-Inhibitor from human placenta exerts ist inhibitory effect by binding non-covalently to RNases in a 1:1 ratio with an association constant of 1014. It is a protein with molecular weight of 51 kDa and inhi-bits common eukaryotic RNases including RNase A, RNase B, RNase C.
It does not inhibit RNase H, S1 Nuclease, SP6, T7 or T3 RNA polymerase, AMV or M-MLV Reverse Transcrip-tase, Taq DNA polymerase and RNase T1.
The enzyme is active over a broad pH range between 5 and 8, with a maximum activity at pH 7-8.

  APPLICATION
Any application where eukaryotic RNase contamination is a potential problem

  • RNA transcription
  • In vitro RNA translation
  • cDNA synthesis
  • DNA and RNA sequencing
  • RT-PCR

  CONCENTRATION
5 units/µl

  UNIT DEFINITION
One unit inhibits 5 ng of RNase A by 50% using cytidine 2',3'-cyclic monophosphate (cCMP) as a substrat.

  STORAGE BUFFER
20 mM Hepes-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol

  STORAGE TEMPERATURE
-20°C

  QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.

  REFERENCES

  1. BLACKBURN, P. (1979) J. Biol. Chem. 254:12484

  



 
 
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