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 100 u




 250 u




 500 u




 1000 u




 5000 u


Native RNase-Inhibitor from human placenta exerts ist inhibitory effect by binding non-covalently to RNases in a 1:1 ratio with an association constant of 1014. It is a protein with molecular weight of 51 kDa and inhi-bits common eukaryotic RNases including RNase A, RNase B, RNase C.
It does not inhibit RNase H, S1 Nuclease, SP6, T7 or T3 RNA polymerase, AMV or M-MLV Reverse Transcrip-tase, Taq DNA polymerase and RNase T1.
The enzyme is active over a broad pH range between 5 and 8, with a maximum activity at pH 7-8.

Any application where eukaryotic RNase contamination is a potential problem

  • RNA transcription
  • In vitro RNA translation
  • cDNA synthesis
  • DNA and RNA sequencing
  • RT-PCR

5 units/µl

One unit inhibits 5 ng of RNase A by 50% using cytidine 2',3'-cyclic monophosphate (cCMP) as a substrat.

20 mM Hepes-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol


Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.


  1. BLACKBURN, P. (1979) J. Biol. Chem. 254:12484


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