GeneCraft - Enzymes for Molecular Biology
Shopping Cart Shopping Cart
Catalog Search  
 
 
 
 
Enzymes Enzymes Enzymes Enzymes Enzymes Enzymes Enzymes     for for for     Molecular Molecular Molecular Molecular Molecular Molecular Molecular Molecular Molecular     Biology Biology Biology Biology Biology Biology Biology
 
  HomeCompanyProductsOrderSupport  
  On-line Catalog  
 
[an error occurred while processing this directive]
  Alphabetical List of Products
 
Thermostable DNA Polymerases
Nucleotides
DNA Molecular Weight Markers
DNA/RNA Modifying Enzymes
Restriction Enzymes
Products for Molecular Biology
Reagents for Electrophoresis
Catalogue
 




  Get Documents
  Downloads
  Find Distributor
 


 
 






    SP6 RNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 SP6 RNA Polymerase

 GC-012-1000  

 1000 u

 45 

 SP6 RNA Polymerase

 GC-012-10000  

 10000 u

 450 



  DESCRIPTION
Isolated from a recombinant E. coli strain this DNA-dependent RNA polymerase is strictly specific for its promoter. It is used to transcribe RNA from DNA templates, cloned into vectors containing SP6 promoter.

  CONCENTRATION
60 units/Ál

  UNIT DEFINITION
One unit is the amount of enzyme that incorporates 1 nmol of labelled nucleoside triphosphates into acid-precipitable RNA in 60 minutes at 37░C.

  STORAGE BUFFER
20 mM Tris-HCl pH 7.5; 100 mM NaCl; 0,1 mM EDTA; 2 mM DTT; 0,1 mg/ml BSA; 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store SP6 RNA polymerase below 0║C, preferably at -20║C, in a constant temperature freezer.

  5X REACTION BUFFER
200 mM Tris-HCl pH 7.9; 30 mM MgCl2; 50 mM DTT

  



 
 
Impressum   |   Shopping Cart