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    T7 RNA polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 T7 RNA polymerase

 GC-020-1000  

 1000 u

 7 

 T7 RNA polymerase

 GC-020-10000  

 10000 u

 70 

 T7 RNA polymerase

 GC-020-20000  

 20000 u

 140 

 T7 RNA polymerase

 GC-020-3000  

 3000 u

 21 

 T7 RNA polymerase

 GC-020-5000  

 5000 u

 35 



  DESCRIPTION
Isolated from recombinant E. coli strain this DNA-dependent RNA polymerase is strictly for its promoter. It is used to transcribe RNA from DNA templates, cloned into vectors containing T7 promoter.

  BESCHREIBUNG
Diese DNA-abhängige RNA-Polymerase ist aus einem rekombinanten E. coli Stamm isoliert worden und absolut spezifisch für den eigenen Promoter. Das Enzym wird verwendet, um RNA von DNA-Templates zu transkribieren, welche in Vektoren kloniert wurden, die den T7-Promoter enthalten.

  CONCENTRATION
60 units/µl

  UNIT DEFINITION
One unit is the amount of enzyme that incorporates 1 nmol of labelled nucleoside triphosphates into acid-precipitable RNA in 60 minutes at 37°C.

  STORAGE BUFFER
20mM Tris-HCl pH 7,5, 100mM NaCl, 0,1mM EDTA, 2mM DTT, 0,1mg/ml BSA, 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store T7 RNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer or near by North Pole.

  5X REACTION BUFFER
200mM Tris-HCl pH 7,9, 30mM MgCl2, 50mM DTT

  



 
 
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