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    Uracil-DNA glycosylase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 Uracil-DNA glycosylase

 GC-038-0100  

 100 u

 20 

 Uracil-DNA glycosylase

 GC-038-0250  

 250 u

 50 

 Uracil-DNA glycosylase

 GC-038-0500  

 500 u

 100 

 Uracil-DNA glycosylase

 GC-038-1000  

 1000 u

 200 

 Uracil-DNA glycosylase

 GC-038-5000  

 5000 u

 1000 



  DESCRIPTION
E. coli uracil-DNA glycosylase (UDG) is the recombinant form of the enzyme, which catalyzes the release of free uracil from uracil-containing DNA (U-DNA) and creates an alkali-sensitive apyrimidinic site in the DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded U-DNA, but not from oligomers (6 or fewer bases).

  APPLICATION

  • PCR
  • RT-PCR
  • site-directed mutagenesis
  • as a probe for protein-DNA interaction studies producing highly labeled oligonucleotide probes

  CONCENTRATION
5 units/µl

  UNIT DEFINITION
One unit of enzyme activity is the amount that catalyzes the degradation of 1 µg single-stranded uracil-con-taining DNA at 37°C in 60 minutes.

  STORAGE BUFFER
20 mM Tris-HCl pH 8.0; 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 0.1 mg/ml BSA; 50% glycerol

  STORAGE TEMPERATURE
Storage temperature -20°C

  10X REACTION BUFFER
500 mM Tricine pH 8.5, 10 mM MgCl2 or buffer for PCR or RT-PCR protocol

  QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.

  



 
 
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