Anti-Taq monoclonal Antibody Clone 1
The Taq Start Monoclonal Antibodies were derived from a hybridoma (fusion of mouse myeloma cell and the cells after mouse immunization with Taq DNA Polymerase). Taq Start Monoclonal Antibody is mouse IgG 2b isotype.
Taq Start Monoclonal Antibody inhibits polymerase activity before the onset of thermal cycling, preventing nonspecific amplification and primer-dimer formation. When the temperature is raised, the antibody is quickly inactivated and PCR proceeds. Taq Start Monoclonal Antibody is effective with a variety of commercially available Taq DNA polymerases (native or recombinant). The use of Taq Start Monoclonal Antibody significantly improves the specificity of PCR amplification what is especially important for PCR-based diagnostics.
- Taq Start PCR
- PCR diagnostics
4 mg/ml (=10 u/µl)
One unit is defined as the amount of Taq Start Monoclonal Antibody required to block 50% of activity of 1 µg of Taq DNA Polymerase at 37°C.
10 mM Tris-HCl (pH 7.0 at 22°C), 50 mM KCl, 0.1 mM EDTA, 50% glycerol
The Taq Start Monoclonal Antibody reaction buffer is the same buffer used for the thermostable DNA polymerase.
More than 95% in SDS electrophoresis in 15% PAAG.
No conversion to the covalently closed circular DNA to the nicked or linear form was observed after incubation of 1 µg of pUC19 with antibodies in final concentration of 6 u/µl in 20 µl of reaction mixture containing 25 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2 after 16 hours at 37°C.
The amount of antibodies required for Taq polymerase activity inhibition depends not on the units of the enzyme, rather the amount of Taq polymerase as protein! The ratio units/mg of Taq polymerase varies strongly from preparation to preparation of the different producers. We recommend to use 2 µl of antibody per 8 µl (40 units) of BioThermTM Taq DNA polymerase.
2500 units of specific activity are equal to 1 mg of antibodies