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    AccuTherm™ DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 AccuTherm™ DNA Polymerase

 GC-004-0100  

 100 u

 20 

 AccuTherm™ DNA Polymerase

 GC-004-0250  

 250 u

 50 

 AccuTherm™ DNA Polymerase

 GC-004-0500  

 500 u

 100 

 AccuTherm™ DNA Polymerase

 GC-004-1000  

 1000 u

 200 

 AccuTherm™ DNA Polymerase

 GC-004-5000  

 5000 u

 1000 



  DESCRIPTION
AccuTherm™ is a thermostable enzyme possessing 5'-3' DNA polymerase and 3'-5' proof reading exonuclea-se activities. It is isolated from the hyperthermophilic marine archae Pyrococcus furiosis (Pfu). AccuTherm™ provides extremely high fidelity. Whereas the enzyme is not able to amplify long fragments as efficiently as BioTherm™ or KlenTherm™ because of its very high exonuclease activity, a mixture of either KlenTherm™ or BioTherm™ with AccuTherm™ provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220).

  CONCENTRATION
5 units/µl

  UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)methyl-amino-propane-sulphonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2, 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCI, 0.5 mM EDTA; 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store AccuTherm™ polymerase below 0°C, preferably at -20°C, in a constant temperature freezer

  COMPANION PRODUCTS
Synergy™ DNA polymerase, SynergyN™ DNA polymerase

  5X REACTION BUFFER
83 mM (NH 4) 2 S0 4 , 350 mM Tris-HCI pH 8.8 (at 25°C), 22 mM MgCl2 , 0,75% Triton X100, 100 mM KCl


  




 
 
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