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    Synergy™ DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 Synergy™ DNA Polymerase

 GC-005-0100  

 100 u

 20 

 Synergy™ DNA Polymerase

 GC-005-0250  

 250 u

 50 

 Synergy™ DNA Polymerase

 GC-005-0500  

 500 u

 100 

 Synergy™ DNA Polymerase

 GC-005-1000  

 1000 u

 200 

 Synergy™ DNA Polymerase

 GC-005-5000  

 5000 u

 1000 



  DESCRIPTION
Synergy is a mix of thermostable components possessing 5'-3' DNA polymerase activity (KlenTherm™ ) and a blend of enzymes (patent pending) improving the accuracy and processivity. These components are optimized to achieve amplification of 35 kb or 10 kb products from lambda templates or genomic DNA, respectively.

  CONCENTRATION
10 units/µl

  UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2 , 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7,0; 100 mM NaCI; 0,5 mM EDTA; 1 mM DTT; 0,01% Tween 20; 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store Synergy™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.

  10X REACTION BUFER
160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2

Please note the difference between Synergy™ and BioTherm™ reaction buffers.

  COMPANION PRODUCTS
SynergyN™ DNA polymerase, AccuTherm™ DNA polymerase.

  TYPICAL REACTION CONDITIONS
TYPICAL REACTION CONDITIONS

  




 
 
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