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    SynergyT™ DNA Polymerase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 SynergyT™ DNA Polymerase

 GC-049-0100  

 100 u

 30 

 SynergyT™ DNA Polymerase

 GC-049-0250  

 250 u

 75 

 SynergyT™ DNA Polymerase

 GC-049-0500  

 500 u

 150 

 SynergyT™ DNA Polymerase

 GC-049-1000  

 1000 u

 300 

 SynergyT™ DNA Polymerase

 GC-049-5000  

 5000 u

 1500 



  DESCRIPTION
Synergy mixture with addition of TthPlus DNA polymerase especially suited for low specificity PCR with degenerated primers.

  APPLICATION

  • Low specificity PCR for degenerated primers

  CONCENTRATION
10 units/µl

  UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)methyl-amino-propanesulphonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2 , 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.

  10X REACTION BUFER
160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2

Please note the difference between Synergy™ and BioTherm™ reaction buffers.

  STORAGE BUFFER
10 mM K-phospate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store SynergyT™ DNA Polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.

  QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nichases and exonucleases.

  REFERENCES

  1. Barnes W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from bacterio-phage templates. Proc. Natl. Acad. Sci. USA 91: 2216-2220

      COMPARISON OF Synergy AND SynergyT UNDER SPECIAL REACTION CONDITIONS
    COMPARISON OF Synergy AND SynergyT UNDER SPECIAL REACTION CONDITIONS

      



 
 
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