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    Klenow fragment

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 Klenow fragment

 GC-009-0100  

 100 u

 12 

 Klenow fragment

 GC-009-0250  

 250 u

 31.25 

 Klenow fragment

 GC-009-0500  

 500 u

 62.5 

 Klenow fragment

 GC-009-1000  

 1000 u

 125 

 Klenow fragment

 GC-009-5000  

 5000 u

 625 



  DESCRIPTION
Klenow fragment is a DNA-dependent polymerase with 3'-5' exonuclease activity. It lacks 5'-3' exonuclease activity of the native enzyme and is suited for random-primed labelling of DNA with random oligonucleotides and for filling-in of 5' protruding ends to blunt-ends.

  CONCENTRATION
5 units/Ál

  UNIT DEFINITION
One unit is the amount of enzyme that incorporates 10 nmoles of deoxynucleotides into acid-insoluble form in 30 minutes at 37░C.

  STORAGE BUFFER
20 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 2 mM DTT, 0.1 mg/ml BSA; 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store Klenow Enzyme below 0║C, preferably at -20║C, in a constant temperature freezer.

  10X REACTION BUFER
100 mM Tris-HCl pH 7.5, 100 mM MgCl2 , 10 mM DTE
(= buffer L for restriction endonucleases!)

  COMPANION PRODUCTS
T4 DNA Ligase, dNTP

  



 
 
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