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    T4 polynucleotide kinase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 T4 polynucleotide kinase

 GC-010-0100  

 100 u

 3.5 

 T4 polynucleotide kinase

 GC-010-0250  

 250 u

 8.75 

 T4 polynucleotide kinase

 GC-010-0500  

 500 u

 17.5 

 T4 polynucleotide kinase

 GC-010-1000  

 1000 u

 35 

 T4 polynucleotide kinase

 GC-010-5000  

 5000 u

 175 



  DESCRIPTION
Isolated from a recombinant E. coli strain this enzyme catalyzes the transfer of the terminal phosphate group of ATP to 5'-OH ends of DNA. T4 polynucleotide kinase is suited for labelling of 5'-OH ends of DNA with ć32P-ATP.

  CONCENTRATION
10 units/µl

  UNIT DEFINITION
One unit is the amount of enzyme that incorporates 1 nmol of acid-insoluble 32P in 30 minutes at 37ºC.

  STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0; 100 mM NaCl; 0.5 mM EDTA; 1 mM DTT; 0.01% Tween 20; 50% glycerol (v/v)

  STORAGE TEMPERATURE
Store T4 polynucleotide kinase below 0ºC, preferably at -20ºC, in a constant temperature freezer.

  5X REACTION BUFFER
350 mM Tris-HCl pH 7,6; 50 mM MgCl2; 25 mM DTT

  



 
 
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