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    Tth DNA Ligase

Products

Cat #

Pack Size  

Price (GBP)  

Qtty

 Tth DNA Ligase

 GC-040-0100  

 100 u

 5 

 Tth DNA Ligase

 GC-040-0250  

 250 u

 12.5 

 Tth DNA Ligase

 GC-040-0500  

 500 u

 25 

 Tth DNA Ligase

 GC-040-1000  

 1000 u

 50 

 Tth DNA Ligase

 GC-040-5000  

 5000 u

 250 



  DESCRIPTION
Thermus thermophilus DNA ligase (recombinant form of the enzyme; cloned from strain HB27) catalyses the formation of a phosphodiester bond between the 5`-phosphate and 3`-hydroxil groups of adjacent nucleotides which are hybridized to a complementary target DNA. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them. There fore, a single-base substitution can be detected.

  APPLICATION

  • LCR DNA
  • ligation

  CONCENTRATION
40 units/µl

  UNIT DEFINITION
One unit is defined as the amount of the enzyme required to give 50% (cohesive end unit) ligation of the 12- base pair cohesive ends of 1 µg of BstE II-digested ë-DNA in 15 minutes at 45°C in a total reaction volume of 50 µl. One cohesive end ligation unit equals 0,015 Weiss units.

  STORAGE BUFFER
10 mM Tris-HCl pH 7.5; 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.2% Triton X-100; 50% glycerol

  STORAGE TEMPERATURE
-20°C

  10X REACTION BUFER
200 mM Tris-HCl pH 7.6; 250 mM KCl; 100 mM MgCl2, 100 mM DTT; 10 mM NAD, 1% Triton X-100
Optimal ligation occurs at 45°C.

  QUALITY CONTROL
Activity, SDS-PAGE purity, absence of RNases, ssDNases, endonucleases and phosphatases.

  



 
 
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